M-MLV is a genetically modified Reverse Transcriptase from Moloney Murine Leukemia Virus. It is different from wild-type M-MuLV in structure, catalytic features and temperature optimum of activity. The enzyme possesses RNA- and DNA-dependent polymerase activity but lacks RNase H activity. Temperature optimum for M-MLV activity is 52°C. The enzyme remains active at temperatures up to 60°C. M-MLV is able to synthesize first strand cDNA up to 10 kb and incorporate modified bases.
Features:
- optimal activity at 52°С
- thermostability till 60°C
- RNAse H-free
- human DNA-free
- extremely low content of E.coli DNA
- any concentration and packing convenient for you
- ambient temperature transportation
- shelf life – up to 24 months
Warm MMLV is modified inactivated M-MLV with increased processivity, activity, and resistance to inhibitors, lack of RNase H. Warm MMLV is activated at 40oС that improves sensitivity of RT-PCR by minimizing or eliminating primer dimer formation and non-specific amplification.
Features:
- activation at 40oС
- optimal activity at 52°С
- thermostability till 60°C
- RNAse H-
- human DNA-free
- extremely low content of E.coli DNA
- any concentration and packing convenient for you
- ambient temperature transportation
- shelf life – up to 24 months
M-MLV is a genetically modified Reverse Transcriptase from Moloney Murine Leukemia Virus. It is different from wild-type M-MuLV in structure, catalytic features and temperature optimum of activity. The enzyme possesses RNA- and DNA-dependent polymerase activity but lacks RNase H activity. Temperature optimum for M-MLV activity is 52°C. The enzyme remains active at temperatures up to 60°C. M-MLV is able to synthesize first strand cDNA up to 10 kb and incorporate modified bases.
Features:
- optimal activity at 52°С
- thermostability till 60°C
- RNAse H-free
- human DNA-free
- extremely low content of coliDNA
- any concentration and packing convenient for you
- ambient temperature transportation
- shelf life - up to 24 months
Warm MMLV is modified inactivated M-MLV with increased processivity, activity, and resistance to inhibitors, lack of RNase H. Warm MMLV is activated at 40oС that improves sensitivity of RT-PCR by minimizing or eliminating primer dimer formation and non-specific amplification.
Features:
- activation at 40oС
- optimal activity at 52°С
- thermostability till 60°C
- RNAse H-
- human DNA-free
- extremely low content of coliDNA
- any concentration and packing convenient for you
- ambient temperature transportation
- shelf life - up to 24 months